11  Appendices

1 Workflow

Table A1: Software used in workflow

step	name	version
Adapter trimming	dorado	v0.6.1
Primer trimming	cutadapt	v4.6
ITS region extraction	ITSxpress	v2.0.1
Quality filtering	chopper	v0.7.0
Chimera detection Clustering Dereplication	VSEARCH	v2.21.1
Subsampling reads	seqtk	v1.4
Dimension reduction	UMAP	v0.4.6
Clustering	HDBSCAN	v0.8.26
Taxonomic assignment	dnabarcoder	v1.0.6
Fastq/fasta file manipulation	seqkit	v2.6.1
Draft read selection	fastANI	v1.34
Read mapping	minimap	v2.28
Read polishing	racon	v1.5.0
Read polishing	medaka	v1.12.0

2 Mock scenarios

Table A2: Read statistics from isolated fungal taxa used in mock scenarios. List of the taxa, sequencing barcode, number of raw reads (following basecalling) and number of reads after quality control (QC). Corrections to original based on the UNITE+INSD (v2024) taxonomy (Abarenkov et al., 2024) are shown in parenthesis.

Barcode	Sample	Number of raw reads	Number of reads after QC
25	Puccinia striiformis var tritici	38,761	9304 (24.0%)
26	Puccinia triticina (Puccinia recondita)e,u	21,731	2216 (10.2%)
27	Puccinia graminis	24,352	6994 (28.7%)
28	Austropuccinia psidii	28,916	8378 (29.0%)
29	Leptosphaeria maculans	40,207	7123 (17.7%)
30	Sclerotinia sclerotiorum	30,922	5461 (17.7%)
31	Botrytis cinerea	35,580	5953 (16.7%)
32	Botrytis fabae	28,674	4533 (15.8%)
33	Zymoseptoria tritici	31,584	2747 (8.7%)
34	Sporisorium scitamineum	29,141	3610 (12.4%)
35	Erysiphe necator	50,836	4889 (9.6%)
36	Austropuccinia psidiiu	29,523	8299 (28.1%)
37	Pyrenophora tritici-repentis	51,246	5655 (11.0%)
38	Cryptovalsa ampelina	37,861	4816 (12.7%)
39	Eutypa lata	25,259	2886 (11.4%)
40	Diplodia seriata	39,280	4821 (12.3%)
41	Fusarium pseudograminearum	37,072	5716 (15.4%)
42	Aspergillus flavum (Aspergillus flavus)	52,132	2775 (5.3%)
43	Aspergillus fumigatus	48,170	4829 (10.0%)
44	Aspergillus nigere,u	106,560	352 (0.3%)
45	Blastobotrys adeninivorans	56,595	11158 (19.7%)
46	Blastobotrys proliferans	45,388	10511 (23.2%)
47	Candida albicans	35,572	6889 (19.4%)
48	candida boletica (Candida boleticola)	45,525	10374 (22.8%)
49	Candida caryicola	42,127	9482 (22.5%)
50	Candida catenulata (Diutina catenulata)	70,924	15285 (21.6%)
51	Candida dublinsiensis (Candida dubliniensis)	62,732	12240 (19.5%)
52	Candida glabrata (Nakaseomyces glabratus)	48,252	5206 (10.8%)
53	Candida metapsilosis	48,123	11117 (23.1%)
54	Candida orthopsilosis	45,771	10222 (22.3%)
55	Candida parapsilosis	53,561	10754 (20.1%)
56	Candida tropicalis	48,486	10111 (20.9%)
57	Candid zeylanoides (Candida zeylanoides)	47,125	11643 (24.7%)
58	Cryptococcus albidus (Naganishia albida)e,u	262	6 (2.3%)
59	Cryptococcus gattil VG I (Cryptococcus gattii VG I)	47,998	9161 (19.1%)
60	Cryptococcus gattii VG IIIu	21,127	3817 (18.1%)
61	Cryptococcus neoformans VNIu	19,970	2842 (14.2%)
62	Cryptococcus neoformans VN IIII	54,596	10852 (19.9%)
63	Fusarium proliferatume,u	79,693	4894 (6.1%)
64	Galactomyces geotrichum (Geotrichum candidum)e,u	15,962	12 (0.1%)
65	Geotrichum candidum	82,477	26203 (31.8%)
66	Kluyveromyces lactis	70,007	14285 (20.4%)
67	Kluyveromyces marxianus	70,959	9212 (13.0%)
68	Kodamaea ohmeri	65,595	11929 (18.2%)
69	Meyerozyma guillermondii (Meyerozyma guilliermondii)e,u	29,370	12 (0.0%)
70	Nakazawaea ernobil (Nakazawaea ernobii)	69,285	15320 (22.1%)
71	Penicillium chrysogenum	64,025	3569 (5.6%)
72	Pichia kudriavzevii	56,683	9807 (17.3%)
73	Pichia membranifacien (Pichia membranifaciens)	39,163	2963 (7.6%)
74	Rhodotorula mucilaginosa	53,680	8580 (16.0%)
75	Scedosporium auranticum (Scedosporium aurantiacum)	52,475	4348 (8.3%)
76	Scedosporium boydii	43,418	4766 (11.0%)
77	Trichomonascus ciferrii	39,999	5338 (13.3%)
78	Trichosporon asahil (Trichosporon asahii)	49,679	11219 (22.6%)
79	Wickerhamomyces anomalus	45,298	8840 (19.5%)
80	Yamadazyma mexicana	44,496	10733 (24.1%)
81	Yamadazyma scolyti	44,166	8874 (20.1%)
82	Yarrowia lipolyticae,u	45,485	10 (0.0%)
83	Diaporthe sp CCL067	36,374	5055 (13.9%)
84	Quambalaria cyanescens CCL055	49,988	6172 (12.3%)
85	Entoleuca sp CCL052	41,661	3845 (9.2%)
86	Cortinarius globuliformis CM4	57,951	7732 (13.3%)
87	Asteroma sp CCL068	45,777	6254 (13.7%)
88	Asteroma sp CCL060	42,061	4449 (10.6%)
89	Tuber brumale	102,909	15169 (14.7%)
e,Excluded from even abundance scenario
uExcluded from uneven abundance scenario

Table A3: Software used basecalling the fungal isolate dataset used in mock scenarios.

step	name	version	command
Basecalling + Demultiplexing	guppy	v6.4.2	guppy_basecaller \  -i ./calledFast5 \  -s ./calledFastq \  -c dna_r10.4.1_e8.2_400bps_sup.cfg \  -r -x auto --disable_qscore_filtering \  --barcode_kits "SQK-NBD114-96" \  --trim_adapters --compress_fastq

2.1 Clustering - NanoCLUST

Figure A1: Chart showing how 58 fungal isolates are clustered into OTUs based on a single execution of the even abundance mock scenario. Each isolate has been subsampled to 2,000 reads and then clustered using the NanoCLUST (UMAP + HDBSCAN) method with a minimum cluster size set to 580 (0.5% of the total library size). Bars indicate the abundance of an OTU (number of reads). The taxonomic classification given by dnabarcoder (with the UNITE 2024 database) for each OTU is shown in the X axis labels (See Table A4 for classifications). Green bars indicate that the assignment given to an OTU matches the expected species-level classification. Yellow bars indicate a correct genus-level classification. Orange bars indicate a correct family-level classification. Red bars indicate the classification is incorrect for family-level and above. Grey indicates that the OTU could not be given a taxonomic classification at all. Bars marked with an asterix (*) indicate that taxonomic labels differed between reference database and our sample but were still considered the same species.

Table A4: The set of taxonomic assignments for each OTU in Figure A1. The most abundant sequence of each OTU was given a taxonomic assignment using dnabarcoder and the UNITE 2024 database. The OTU ID column refers OTUs found in Figure A1 (x-axis). The genus assignment, species assignment, BLAST score and confidence as reported by dnabarcoder are also shown.

OTU ID	genus	species	score	confidence
0	Nakazawaea	Nakazawaea ernobii	100.00%	83.87%
1	Tuber	Tuber brumale	99.88%	90.68%
2	Fusarium	Fusarium pseudograminearum	100.00%	82.32%
3	Candida	Candida albicans	100.00%	92.48%
4	Candida	Candida tropicalis	100.00%	92.48%
5	Cryptovalsa	Cryptovalsa ampelina	100.00%	82.32%
6	unclassified kingdom	unclassified kingdom	78.00%	0.00%
7	unclassified kingdom	unclassified kingdom	79.25%	0.00%
8	Dipodascus	Dipodascus capitatus	90.50%	90.87%
9	Eutypa	Eutypa lata	100.00%	93.14%
10	Puccinia	Puccinia graminis	100.00%	82.32%
11	Botrytis	Botrytis genus	100.00%	74.10%
12	Inopinatum	Inopinatum lactosum	100.00%	82.32%
13	Candida	Candida dubliniensis	100.00%	92.48%
14	unclassified kingdom	unclassified kingdom	86.00%	0.00%
15	Cryptococcus	Cryptococcus neoformans	100.00%	93.32%
16	Trichosporon	Trichosporon asahii	100.00%	82.32%
17	Pichia	Pichia genus	95.75%	82.49%
18	Yamadazyma	Yamadazyma mexicana	100.00%	86.96%
19	Blastobotrys	Blastobotrys adeninivorans	100.00%	95.61%
20	Yamadazyma	Yamadazyma scolyti	100.00%	86.96%
21	Candida	Candida parapsilosis	100.00%	92.48%
22	Candida	Candida africana	100.00%	92.48%
23	Aspergillus	Aspergillus flavus	100.00%	90.31%
24	Scedosporium	Scedosporium aurantiacum	100.00%	82.32%
25	Cortinarius	Cortinarius genus	99.50%	57.27%
26	Wickerhamomyces	Wickerhamomyces anomalus	100.00%	96.38%
27	Debaryomycetaceae family	Debaryomycetaceae family	100.00%	73.25%
28	Entoleuca	Entoleuca genus	100.00%	57.27%
29	Gnomoniopsis	Gnomoniopsis paraclavulata	100.00%	95.16%
30	Diaporthe	Diaporthe foeniculina	100.00%	82.32%
31	Penicillium	Penicillium rubens	100.00%	90.71%
32	Erysiphe	Erysiphe necator	100.00%	89.16%
33	Rhodotorula	Rhodotorula mucilaginosa	100.00%	82.32%
34	Kurtzmaniella	Kurtzmaniella genus	100.00%	73.25%
35	Quambalaria	Quambalaria cyanescens	100.00%	82.32%
36	unclassified kingdom	unclassified kingdom	87.50%	0.00%
37	Puccinia	Puccinia striiformis	100.00%	82.32%
38	Sclerotinia	Sclerotinia sclerotiorum	100.00%	96.69%
39	Cortinarius	Cortinarius genus	99.67%	57.27%
40	Trichomonascus	Trichomonascus genus	100.00%	57.27%
41	Cryptococcus	Cryptococcus gattii	100.00%	93.32%
42	Pyrenophora	Pyrenophora tritici-repentis	100.00%	93.15%
43	Leptosphaeria	Leptosphaeria maculans	100.00%	91.09%
44	Scedosporium	Scedosporium boydii	100.00%	82.32%
45	Blastobotrys	Blastobotrys proliferans	100.00%	95.61%
46	Sporisorium	Sporisorium scitamineum	100.00%	90.16%
47	Puccinia	Puccinia graminis	100.00%	82.32%
48	Nakaseomyces	Nakaseomyces genus	100.00%	80.41%
49	Puccinia	Puccinia psidii	100.00%	82.32%
50	Zymoseptoria	Zymoseptoria tritici	100.00%	82.32%
51	Candida	Candida orthopsilosis	100.00%	92.48%
52	Kluyveromyces	Kluyveromyces marxianus	100.00%	82.32%
53	Komagataella	Komagataella pastoris	100.00%	82.32%
54	Pichia	Pichia kudriavzevii	100.00%	86.66%
55	Candida	Candida orthopsilosis	99.76%	92.48%
56	Diplodia	Diplodia seriata	100.00%	93.98%
57	Puccinia	Puccinia psidii	100.00%	82.32%

2.2 Clustering - VSEARCH

Figure A2: Chart showing how 58 fungal isolates are clustered into OTUs based on a single execution of the even abundance mock scenario. Each isolate has been subsampled to 2,000 reads and then clustered using VSEARCH at 97% identity. OTUs with less than 174 reads were removed (0.15% of the total library size). Bars indicate the abundance of an OTU (number of reads). The taxonomic classification given by dnabarcoder (with the UNITE 2024 database) for each OTU is shown in the X axis labels (See Table A5 for classifications). Green bars indicate that the assignment given to a OTU matches the expected species-level classification. Yellow bars indicate a correct genus-level classification. Orange bars indicate a correct family-level classification. Red bars indicate the classification is incorrect for family-level and above. Grey indicates that the OTU could not be given a taxonomic classification at all. Bars marked with an asterix (*) indicate that taxonomic labels differed between reference database and our sample but were still considered the same species.

Table A5: The set of taxonomic assignments for each OTU in Figure A2. The most abundant sequence of each OTU was given a taxonomic assignment using dnabarcoder and the UNITE 2024 database. The OTU ID column refers to OTUs found in Figure A2 (x-axis). The genus assignment, species assignment, BLAST score and confidence as reported by dnabarcoder are also shown.

OTU ID	genus	species	score	confidence
1	Cryptococcus	Cryptococcus gattii	99.88%	90.68%
2	Penicillium	Penicillium rubens	100.00%	82.32%
3	Eutypa	Eutypa lata	100.00%	92.48%
4	unclassified kingdom	unclassified kingdom	100.00%	92.48%
5	Puccinia	Puccinia psidii	100.00%	82.32%
6	Trichomonascus	Trichomonascus genus	78.00%	0.00%
7	Yamadazyma	Yamadazyma scolyti	79.25%	0.00%
8	Puccinia	Puccinia graminis	90.50%	90.87%
9	Cryptococcus	Cryptococcus neoformans	100.00%	93.14%
10	Erysiphe	Erysiphe necator	100.00%	82.32%
11	Yamadazyma	Yamadazyma mexicana	100.00%	74.10%
12	Trichosporon	Trichosporon asahii	100.00%	82.32%
13	Botrytis	Botrytis genus	100.00%	92.48%
14	unclassified kingdom	unclassified kingdom	86.00%	0.00%
15	Sporisorium	Sporisorium scitamineum	100.00%	93.32%
16	Pyrenophora	Pyrenophora tritici-repentis	100.00%	82.32%
17	Kluyveromyces	Kluyveromyces marxianus	95.75%	82.49%
18	Wickerhamomyces	Wickerhamomyces anomalus	100.00%	86.96%
19	Pichia	Pichia genus	100.00%	95.61%
20	unclassified kingdom	unclassified kingdom	100.00%	86.96%
21	Fusarium	Fusarium pseudograminearum	100.00%	92.48%
22	Cryptovalsa	Cryptovalsa ampelina	100.00%	92.48%
23	Tuber	Tuber brumale	100.00%	90.31%
24	Inopinatum	Inopinatum lactosum	100.00%	82.32%
25	Candida	Candida albicans	99.50%	57.27%
26	Candida	Candida dubliniensis	100.00%	96.38%
27	Blastobotrys	Blastobotrys proliferans	100.00%	73.25%
28	Candida	Candida parapsilosis	100.00%	57.27%
29	Scedosporium	Scedosporium aurantiacum	100.00%	95.16%
30	Entoleuca	Entoleuca genus	100.00%	82.32%
31	Leptosphaeria	Leptosphaeria maculans	100.00%	90.71%
32	Aspergillus	Aspergillus flavus	100.00%	89.16%
33	Sclerotinia	Sclerotinia sclerotiorum	100.00%	82.32%
34	Cortinarius	Cortinarius genus	100.00%	73.25%
35	Cryptococcus	Cryptococcus gattii	100.00%	82.32%
36	Blastobotrys	Blastobotrys adeninivorans	87.50%	0.00%
37	Nakazawaea	Nakazawaea ernobii	100.00%	82.32%
38	Rhodotorula	Rhodotorula mucilaginosa	100.00%	96.69%
39	Zymoseptoria	Zymoseptoria tritici	99.67%	57.27%
40	unclassified kingdom	unclassified kingdom	100.00%	57.27%
41	Kurtzmaniella	Kurtzmaniella genus	100.00%	93.32%
42	Dipodascus	Dipodascus capitatus	100.00%	93.15%
43	Quambalaria	Quambalaria cyanescens	100.00%	91.09%
44	Diplodia	Diplodia seriata	100.00%	82.32%
45	Puccinia	Puccinia striiformis	100.00%	95.61%
46	Debaryomycetaceae family	Debaryomycetaceae family	100.00%	90.16%
47	Gnomoniopsis	Gnomoniopsis paraclavulata	100.00%	82.32%
48	Candida	Candida orthopsilosis	100.00%	80.41%
49	Candida	Candida tropicalis	100.00%	82.32%
50	Komagataella	Komagataella pastoris	100.00%	82.32%
51	Diaporthe	Diaporthe foeniculina	100.00%	92.48%
52	Scedosporium	Scedosporium boydii	100.00%	82.32%
53	Nakaseomyces	Nakaseomyces genus	100.00%	82.32%
54	unclassified kingdom	unclassified kingdom	100.00%	86.66%

3 Real world - Soil case study

Table A6: Read statistics for soil sample data. List of the samples, number of raw reads (following basecalling) and number of reads after quality control (QC).

Sample	Number of raw reads	Number of reads after QC
AL1	239,709	186363 (77.7%)
AL2	231,117	152282 (65.9%)
AL3	186,404	137353 (73.7%)
AL4	118,352	91943 (77.7%)
AL5	73,496	53703 (73.1%)
AL6	147,981	93942 (63.5%)
AP1	71,005	51227 (72.1%)
AP2	103,684	69442 (67.0%)
AP3	97,940	69235 (70.7%)
AP4	114,140	84609 (74.1%)
AP5	144,789	90968 (62.8%)
AP6	3,577	1173 (32.8%)
EC1	29,232	17466 (59.7%)
EC2	72,957	55584 (76.2%)
EC3	122,122	85317 (69.9%)
EC5	111,698	72093 (64.5%)
EC6	104,171	79082 (75.9%)
EV1	98,575	73681 (74.7%)
EV2	76,320	51884 (68.0%)
EV3	66,069	49847 (75.4%)
EV4	94,882	64587 (68.1%)
EV5	111,569	70090 (62.8%)
EV6	215,888	138717 (64.3%)
ExCon	334,396	6672 (2.0%)
PCRNegCon	71,377	2400 (3.4%)

Table A7: Software used basecalling and demultiplexing the soil sample dataset

step	name	version	command
Basecalling	dorado	v0.7.1
Demultiplexing	minibar	v0.25	minibar.py -F \  ../minibar-primers.txt \  ../raw/SoilSamples.fastq.gz

Table A8: Top 30 largest OTUs in the soil case study dataset after clustering the full set of reads (~3 million) with VSEARCH at 97% identity. Each column shows: the classification given by dnabarcoder, the number of reads in the OTU, the BLAST identity score for the classification, the percent identity cutoff used by dnabarcoder, the UNITE+INSD reference identifier of the closest match, and the name of the closest match from the UNITE+INSD database.

classification	count	score	cutoff	ReferenceID	closest match
unclassified kingdom	239,241	90.06%		KC152167	Geastrum genus
Archaeorhizomyces genus	41,602	95.86%	95.60%	UDB01263360	Archaeorhizomyces genus
Tomentella genus	38,361	99.83%	95.60%	UDB06025650	Tomentella genus
unclassified kingdom	37,311	93.75%		GU222314	Amanita australis
Laccaria genus	35,455	99.83%	95.60%	OQ064943	Laccaria genus
unclassified kingdom	34,909	87.87%		KP889944	Archaeorhizomycetes class
Mortierella genus	33,161	100.00%	95.60%	UDB04902775	Mortierella genus
Clavulina genus	32,208	100.00%	95.60%	DQ672317	Clavulina genus
Scleroderma genus	25,070	98.74%	95.60%	UDB06676397	Scleroderma genus
unclassified kingdom	23,785	88.16%		UDB04632058	Piloderma genus
unclassified kingdom	22,597	92.49%		DQ309136	Rozellomycota phylum
Hydnodontaceae family	21,074	94.53%	93.80%	OL828784	Trechispora praefocata
Oidiodendron genus	17,240	100.00%	95.60%	OK584555	Oidiodendron genus
Leucoagaricus genus	16,640	99.51%	95.60%	UDB03049341	Leucoagaricus genus
unclassified kingdom	16,578	49.28%		UDB0760917	Inocybe genus
unclassified kingdom	16,310	48.77%		UDB0343814	Helotiales order
unclassified kingdom	15,946	90.83%		DQ474677	Piloderma genus
Penicillium genus	15,849	100.00%	95.30%	UDB04263718	Penicillium genus
Amanita genus	15,823	96.85%	95.60%	GU222314	Amanita australis
unclassified kingdom	15,541	93.17%		UDB01262630	Archaeorhizomyces genus
Aspergillus felis	11,912	100.00%	99.20%	OQ152598	Aspergillus felis
Tomentella genus	10,887	100.00%	95.60%	UDB06165394	Tomentella genus
unclassified kingdom	9,990	58.30%		UDB0756057	Sebacina genus
unclassified kingdom	9,824	36.89%		UDB0757809	Pyronemataceae family
Talaromyces genus	9,195	100.00%	93.70%	OP497912	Talaromyces genus
unclassified kingdom	8,996	58.30%		UDB0756057	Sebacina genus
unclassified kingdom	8,761	0.00%
Penicillium genus	8,737	100.00%	95.30%	UDB03940467	Penicillium genus
Inocybe subferruginea	8,729	99.37%	99.10%	KP636874	Inocybe subferruginea
unclassified kingdom	8,547	93.27%		UDB0761652	Cortinarius genus

Table A9: Top 30 largest OTUs in the soil case study dataset after clustering the full set of reads (~3 million) with NanoCLUST. Each column shows: the classification given by dnabarcoder, the number of reads in the OTU, the BLAST identity score for the classification, the percent identity cutoff used by dnabarcoder, the UNITE+INSD reference identifier of the closest match, and the name of the closest match from the UNITE+INSD database.

classification	count	score	cutoff	ReferenceID	closest match
Penicillium genus	14,879	100.00%	95.30%	UDB03808858	Penicillium genus
unclassified kingdom	3,191	90.24%		KC152167	Geastrum genus
Laccaria genus	2,143	99.83%	95.60%	UDB02413463	Laccaria genus
unclassified kingdom	2,069	93.75%		GU222314	Amanita australis
Mortierella genus	1,904	100.00%	95.60%	UDB04902775	Mortierella genus
unclassified kingdom	1,816	90.42%		UDB02700124	Geastrum genus
Archaeorhizomyces genus	1,418	95.86%	95.60%	UDB01263360	Archaeorhizomyces genus
unclassified kingdom	1,263	90.06%		KC152167	Geastrum genus
unclassified kingdom	1,219	92.49%		DQ309136	Rozellomycota_gen_Incertae_sedis genus
Oidiodendron genus	1,036	100.00%	95.60%	OK584555	Oidiodendron genus
Penicillium citrinum	1,023	100.00%	99.20%	MN783029	Penicillium citrinum
Penicillium genus	960	100.00%	95.30%	UDB04263718	Penicillium genus
Clavulina genus	905	100.00%	95.60%	DQ672317	Clavulina genus
Scleroderma genus	846	98.74%	95.60%	UDB06676397	Scleroderma genus
unclassified kingdom	842	49.28%		UDB0760917	Inocybe genus
Aspergillus felis	827	100.00%	99.20%	OQ152598	Aspergillus felis
Tomentella genus	810	100.00%	95.60%	UDB06171495	Tomentella genus
Leucoagaricus genus	636	99.51%	95.60%	UDB03049341	Leucoagaricus genus
Amanita genus	629	96.85%	95.60%	GU222314	Amanita australis
Talaromyces genus	614	100.00%	93.70%	OP497912	Talaromyces genus
Hydnodontaceae family	588	94.53%	93.80%	OL828784	Trechispora praefocata
Leucoagaricus genus	579	99.67%	95.60%	UDB03049341	Leucoagaricus genus
Penicillium genus	571	100.00%	95.30%	OQ870808	Penicillium genus
unclassified kingdom	566	90.45%		UDB06347141	Trechispora genus
Hydnodontaceae family	556	94.73%	93.80%	OL828784	Trechispora praefocata
Hydnaceae family	548	95.04%	93.80%	UDB07049378	Sistotrema genus
unclassified kingdom	512	93.27%		UDB0761652	Cortinarius genus
unclassified kingdom	500	90.83%		DQ474677	Piloderma genus
unclassified kingdom	465	90.66%		KC152167	Geastrum genus
Meyerozyma guilliermondii	440	100.00%	99.10%	OR761551	Meyerozyma guilliermondii

Abarenkov,K. et al. (2024) Full UNITE+INSD dataset for Fungi.